清华大学化学系梁琼麟

lianmeng

梁琼麟，清华大学化学系长聘教授，研究方向为药物分析与生命分析科学。以微流控芯片及其与质谱、光谱联用分析技术为基础，发展生命分析与药物分析新方法，开发生物医用新材料新器件，发明器官类器官芯片新模型，致力于服务国家药品质量与安全、新药创制以及中药现代化研究与开发。近年来重点聚焦于器官类器官芯片、单细胞亚细胞分析及基于质谱的多组学分析等。曾主持完成国家重大科技专项第一个微流控芯片药物研发关键技术项目，在器官芯片核心关键技术及血管、肝、肾、肠等器官芯片模型研究方面取得重要进展。2016-2020年间以第一发明人申请发明专利16项（授权8项），以通讯作者在Nat. Protoc., Adv. Mater., Anal. Chem., Lab Chip等重要学术期刊上发表SCI论文59篇（14篇IF>10）。工作以来累计发表SCI论文180余篇，Web of Science引用5000余次，发明专利30余项。部分研究成果已在制药企业、临床医院得到广泛应用，曾合作获得国家科技进步二等奖3项。


梁琼麟   博士  教授 博士生导师
Tel.  86-10-62772263
Fax.  86-10-62772263
Email: LIANGQL@tsinghua.edu.cn
教育背景
1996 - 2000    清华大学化学系 化学专业     理学学士学位
2000 - 2005    清华大学化学系 分析化学专业 理学博士学位
工作履历
2005.8 - 至今， 清华大学化学系 讲师，副教授
学术兼职
中国医药生物技术协会生物制药技术专业委员会常务委员，学术秘书
中国医药生物技术协会生物芯片专业委员会委员
Journal of Integrated Omics, 编委
研究领域
生命分析化学(研究方向：生物微流控、生物质谱)
研究概况
在分析化学与生命科学的交叉领域，致力于发展创新的分析原理、试验方法及仪器装置，参与生命科学重大基础研究，服务于药物研发、临床检测等国家重大需求。针对生物复杂系统体外（in vitro）-体内（in vivo）研究的不同需求，主要开辟两个研究方向：一是基于微流控等新技术构建模拟体内微环境和生物过程的体外研究平台；二是基于生物质谱和纳米技术构建复杂生物样品的组学分析平台和体内原位实时分析方法。近五年主持承担了国家重大科技专项重大课题，国家973子课题以及国家自然科学基金青年和面上项目等多项国家科研任务。在SCI期刊上发表论文80余篇，其中影响因子5以上论文15篇，SCI引用1200余次。以共同作者出版学术著作5部, 获得授权中国发明专利8项。曾应邀在美国、比利时、德国、日本、韩国等多次国际会议上做学术报告并获青年科学家报告奖。研究成果合作获得了国家科技进步二等奖以及省部级科技进步一等奖3项、二等奖2项，中国分析测试协会一等奖1项。
奖励与荣誉
2013 教育部新世纪优秀人才支持计划
2012 北京市科技进步二等奖（4/10）
2012 河北省科技进步一等奖（7/10）
2011 上海市科技进步一等奖（12/15）
2011 The PSC (Permanent Scientific Committee of HPLC Symposium) Young Scientist Lecture Award
2010 河北省医药科学技术进步一等奖（6/10）
2009 清华大学“先进工作者”称号
2007 国家科学技术进步二等奖(7/10)
2005 教育部科技进步一等奖及中华中医药学会一等奖(9/15)  
2005 中华中医药学会科学技术二等奖（7/10）
2004 中国分析测试协会科学技术一等奖(4/4)
学术成果
参与出版专著：
1.  Guoan Luo, Yiming Wang, Qionglin Liang, Qingfei Liu. 《Systems Biology for Traditional Chinese Medicine》, Wiley, NJ USA, 2012
2.  罗国安，王义明，梁琼麟，刘清飞.《中医药系统生物学》（99.9万字），科学出版社，北京，2010
3.  罗国安，梁琼麟，王义明.《中药指纹图谱: 质量评价、质量控制与新药研发》（101万字），化工出版社，北京，2009
4.  罗国安，卓先义，陈令新，梁琼麟.《药物与毒物分析技术》（59.5万字），化学工业出版社，北京，2007
5.  罗国安，王义明，陈令新，梁琼麟.《毛细管电色谱及其在生命科学中的应用》（26.7万字），科学出版社，北京，2005
近三年代表性论文（Selected Recent Publications）：
1. Liang XP, Wang Y, Liang QL*, Wang YM, Huang M and Luo GA*. Pathogenesis of neural tube defects: the story beyond methylation or one-carbon unit metabolism. Metabolomics  2012 published on line DOI 10.1007/s11306-011-0387-z
2. Liang QL, Liang XP, Wang YM, Xie YY, Zhang RL, Chen X, Gao R, Cheng YJ, Wu J, Xu QB, Xiao QZ, Li X, Lv SF,  Fan XM,  Zhang HY,  Zhang QL, Luo GA. Effective components screening and anti-myocardial infarction mechanism study of the Chinese medicine NSLF6 based on "system to system" mode. Journal of Translational Medicine 2012, 10(26): 1-10
3. Cong WJ, Liang QL ; Li L ; Shi J ; Liu QF ; Feng Y ; Wang YM; Luo GA.Metabonomic study on the cumulative cardiotoxicity of a pirarubicin liposome powder. Talanta, 2012, 89: 91-98
4. Zhang K, Liang QL*, Ai XN, Hu P, Wang YM, Luo GA*.  Comprehensive two-dimensional manipulations of picoliter microfluidic droplets sampled from nanoliter samples. Analytical Chemistry 2011, 83(20) :8029-8034.
5. Liang XP, Chen X, Liang QL*, Wang YM, Luo GA*. Metabonomic study of Chinese medicine Shuanglong Formula as an effective treatment for myocardial infarction in rats. Journal of Proteome Research,  2011, 10, 790–799
6. Han LD, Xia JF, Liang QL*, Wang Y, Hu P, Luo GA*.  Plasma esterified and non-esterified fatty acids metabolic profiling using gas chromatography - mass spectrometry and its application in the study of diabetic mellitus and diabetic nephropathy, Analytica Chimica Acta 2011, 689, 85-91
7. Zhang K, Liang QL*, Ai XN, Hu P, Wang YM, Luo GA*.  On-demand microfluidic droplet manipulation using hydrophobic ferrofluid as a continuous-phase. Lab on a Chip, 2011, 11 (7), 1271 – 1275
8. Zhu C, Liang QL*, Hu P, Wang YM, Luo GA*.  Phospholipidomic identification of potential plasma biomarkers associated with type 2 diabetes mellitus and diabetic nephropathy. Talanta 2011, 85(4):1711-1720
9. He TX, Liang QL*, Zhang K, Mu X, Luo TT, Wang YM, Luo GA. A modified microfluidic chip for fabrication of paclitaxel-loaded poly(L-lactic acid) microspheres. Microfluidics and Nanofluidics, 2011, 10(6): 1289-1298  
10. Jiang ZT, Sun JB, Liang QL, Cai YF, Li SS, Huang Y, Wang YM, Luo GA*.  A Metabonomic Approach Applied to Predict Patients with Cerebral Infarction. Talanta, 2011, 84: 298-304
11. Cheng YJ, Zhang M, Liang QL*, Hu P, Wang Y, Wu F.J, LuoGA*.  Two-step Preparation of Ginsenoside-Re, Rb1, Rc and Rb2 from the Root of Panax ginseng by High-Performance Counter-current Chromatography. Separation and Purification Technology, 2011, 77 : 347-354
12. Zhang, M; Ignatova, S; Hu, P; Liang, QL; Wang, YM; Luo, GA; Jun, FW; Sutherland, I. Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study. Journal of Chromatography A  , 2011, 1218(36): 6031-6037
13. Zhang, K; Liang, QL*; Ma, S; He, TX; Ai, XN; Hu, P; Wang, YM; Luo, GA*. A gravity-actuated technique for flexible and portable microfluidic droplet manipulation. Microfluidics and Nanofluidics 2010, 9 (4-5): 995-1001
14. Jiang, ZT; Liang, QL; Wang, Y; Zheng, XY; Pei, LJ; Zhang, T; Wang, YM; Luo, GA*. Metabonomic study on women of reproductive age treated with nutritional intervention: screening potential biomarkers related to neural tube defects occurrence. Biomedical Chromatography , 2011, 25( 7): 767-774
15. He, TX; Liang, QL*; Luo, TT; Wang, YM; Luo, GA*. Study on Interactions of Phenolic Acid-Like Drug Candidates with Bovine Serum Albumin by Capillary Electrophoresis and Fluorescence Spectroscopy. Journal of Solution Chemistry 2010,39 (11): 1653-1664
16. Cheng, YJ; Liang, QL*; Hu, P; Wang, YM; Jun, FW; Luo, GA*. Combination of normal-phase medium-pressure liquid chromatography and high-performance counter-current chromatography for preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency. Separation and Purification Technology 2010,73 (3): 397-402
17. Qi, SQ; Pang, YX; Hu, Q; Liu, Q; Li, H; Zhou, YL; He, TX; Liang, QL; Liu, YX; Yuan, XQ; Luo, G; Li, HL; Wang, JW; Yan, N; Shi, YG. Crystal Structure of the Caenorhabditis elegans Apoptosome Reveals an Octameric Assembly of CED-4.  CELL 2010,141 (3): 446-457
18. Xia, JF; Hu, P; Liang, QL*; Zou, TT; Wang, YM; Luo, GA*.  Correlations of creatine and six related pyrimidine metabolites and diabetic nephropathy in Chinese type 2 diabetic patients. Clinical Biochemistry 2010,43 (12): 957-962
19. Mu, XA; Liang, QL*; Hu, P; Ren, KN; Wang, YM; Luo, GA*.  Selectively modified microfluidic chip for solvent extraction of Radix Salvia Miltiorrhiza using three-phase laminar flow to provide double liquid-liquid interface area. Microfluidics and Nanofluidics 2010, 9 (2-3): 365-373
20. Qi, XC; Ignatova, S; Luo, GA; Liang, QL; Jun, FW; Wang, YM; Sutherland, I. Preparative isolation and purification of ginsenosides Rf, Re, Rd and Rb1 from the roots of Panax ginseng with a salt/containing solvent system and flow step-gradient by high performance counter-current chromatography coupled with an evaporative light scattering detector. Journal of Chromatography A 2010,1217 (13): 1995-2001
21. He, TX; Liang, QL*; Wang, YM; Luo, GA. Characterization of the Interactions between Natural Flavonoid Compounds and Bovine Serum Albumin by Capillary Electrophoresis Aand Fluorescence Method. Journal of Liquid Chromatography & Related Technologies 2010,33 (4): 548-562
22. Ren, KN; Liang, QL*; Mu, X; Luo, GA*; Wang, YM. Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source. Lab on a Chip 2009, 9 (5): 733-736.
23. Chen, J; Liang, QL*; Hua, HY; Wang, YM; Luo, GA*; Hu, MQ; Na, YQ. Simultaneous determination of 15 steroids in rat blood via gas chromatography-mass spectrometry to evaluate the impact of emasculation on adrenal. Talanta 2009, 80 (2): 826-832
24. Zhang, K; Liang, QL*; Ma, S; Mu, XA; Hu, P; Wang, YM; Luo, GA*. On-chip manipulation of continuous picoliter-volume superparamagnetic droplets using a magnetic force. Lab on a Chip 2009, 9 (20): 2992-2999.
25. Mu, X; Liang, QL*; Hu, P; Ren, KN; Wang, YM; Luo, GA*.  Laminar flow used as "liquid etch mask" in wet chemical etching to generate glass microstructures with an improved aspect ratio. Lab on a Chip 2009, 9 (14): 1994-1996
26. Liang, XP; Liang, QL*; Xia, JF; Wang, Y; Hu, P; Wang, YM; Zheng, XY; Zhang, T; Luo, GA*. Simultaneous determination of sixteen metabolites related to neural tube defects in maternal serum by liquid chromatography coupling with electrospray tandem mass spectrometry. Talanta 2009, 78 (4-5): 1246-1252.
27. Mu, X; Liang, QL*; Hu, P; Yao, B; Ren, KN; Wang, YM; Luo, GA*. Prototypical Nonelectrochemical Method for Surface Regeneration of an Integrated Electrode in a PDMS Microfluidic Chip. Analytical Letters 2009, 42 (13): 1986-1996.
28. Zhang, HY; Chen, X; Hu, P; Liang, QL; Liang, XP; Wang, YM; Luo, GA. Metabolomic profiling of rat serum associated with isoproterenol-induced myocardial infarction using ultra-performance liquid chromatography/time-of-flight mass spectrometry and multivariate analysis. Talanta 2009, 79 (2): 254-259.
29. Xia, JF; Liang, QL*; Liang, XP; Wang, YM; Hu, P; Li, P; Luo, GA*. Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2009, 877 (20-21): 1930-1936.
30. Zhang, M; Ignatova, S; Liang, QL; Jun, FW; Sutherland, I; Wang, YM; Luo, GA. Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography. Journal of Chromatography A 2009, 1216 (18): 3869-3873.
31. Xia, JF; Liang, QL; Hu, P; Wang, YM; Li, P; Luo, GA. Correlations of six related purine metabolites and diabetic nephropathy in Chinese type 2 diabetic patients. Clinical Biochemistry 2009, 42 (3): 215-220.
32. Jiang, ZT; Liang, QL; Luo, GA; Hu, P; Li, P; Wang, YM. HPLC-electrospray tandem mass spectrometry for simultaneous quantitation of eight plasma aminothiols: Application to studies of diabetic nephropathy. Talanta 2009, 77 (4): 1279-1284.

xibei

活性氧（ROS）在体内通过生物代谢过程持续不断的产生。作为必需的次级信使物质，ROS对调节生物体的生理功能起着重要的作用。然而，不受控产生过量的ROS会引起氧化应激效应，从而对正常的组织器官造成不可逆转的损伤。为了保护生物体免受氧化应激诱导的炎症困扰，开发新型抗氧化剂清除过量的ROS以维持细胞内的氧化还原平衡是一个重要的研究内容。随着纳米技术的发展，模块化设计的功能材料不断的涌现。制备模块化组装的高度集成纳米复合材料以模拟抗氧化酶快速高效的清除胞内过多的ROS具有重要的研究价值。

        面对清除过量ROS的迫切需求，受天然多酶系统的启发，清华大学梁琼麟教授等合成了一种高度集成的核-壳结构纳米复合材料。通过简单的自由基聚合反应和原位氧化还原反应方法，制备了多壳二氧化锰包裹硒-聚多巴胺（Se@Me@MnO2）纳米复合材料。体外实验结果表明，Se@Me@MnO2纳米复合材料是一种高效的多酶模拟物。它可以类通过过氧化氢酶（CAT），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的催化机制清除H2O2，•OH和O2•-以实现细胞内抗氧化效果。进一步的机理研究表明，Se核具有类GPx的酶催化活性，MnO2和Me具有类SOD和类CAT的酶催化活性。动物模型实验表明，有害的氧化应激得到有效抑制，炎症得到明显缓解。所开发的ROS清除系统具有以下优势：（1）Se@Me@MnO2纳米酶对可以高效的清除多种ROS（H2O2，HO•和O2•-）；（2）Se@Me@MnO2纳米酶具有CAT，SOD和GPx的抗氧化能力；（3）能够有效缓解炎症反应引发的ROS；（4）Se@Me@MnO2纳米酶具有良好的生物相容性。以上结果表明，新开发的Se@Me@MnO2纳米酶在胞内抗氧化及对ROS相关疾病的治疗具有应用前景。


        文章信息:Yongjian Ai, Jinzhi You, Jianyi Gao, Jiaping Wang, Hong-bin Sun, Mingyu Ding, Qionglin Liang*. Multi-shell nanocomposites based multienzyme mimetics for efficient intracellular antioxidation. Nano Res., 2020 https://doi.org/10.1007/s12274-020-3267-x.