天津大学化工学院陈磊

tinengyue

陈　磊
现任职称/职务：制药工程系副教授/博导
通讯地址：天津市南开区卫津路92号天津大学化工学院
电子邮箱：lchen@tju.edu.cn
办公电话：022-27406364
研究方向:
微生物合成生物学；光合蓝细菌；微藻；分子生物学；系统生物学；信号转导系统


讲授课程:
《分子与合成生物学》、《生物化工导论》
学术兼职:
任Frontiers in Microbiology审稿编辑（Review Editor)。Frontiers in Microbiology, International Journal of Hydrogen Energy, Journal of Molecular Microbiology and Biotechnology, PloS one等多个国际学术刊物的经常评审人。
学术成就:
在国际学术刊物发表SCI论文70余篇，其中第一及通讯作者SCI论文30余篇。
科研项目:
主持国家自然科学基金面上项目3项、天津市自然科学基金青年基金1项、天津大学自主创新基金多项，参与国家“973计划”项目3项、“863计划“项目1项，累计主持研究经费400多万元。
论著专利:
(1)    Pei, G., Li, X., Liu, L., Liu, J., Wang, F., Chen, L., Zhang W. De novo transcriptomic and metabolomic analysis of docosahexaenoic acid (DHA) - producing Crypthecodinium cohnii during fed-batch fermentation. Algal Research 2017, Accepted.
(2)    Sun, T., Xu, L., Wu, L., Song, Z., Chen, L.*, Zhang, W. Identification of a new target slr0946 of the response regulator Sll0649 involving cadmium tolerance in Synechocystis sp. PCC 6803. Frontiers in Microbiology 2017, Accepted. doi: 10.3389/fmicb.2017.01582
(3)    Chen, Z., Chen, L.*, Zhang, W. Tools for genomic and transcriptomic analysis of microbes at single-cell level. Frontiers in Microbiology 2017, In review. (IF=4.165, 二区)
(4)    Liu, J, Pei, G., Diao, J., Chen, Z., Liu, L., Chen, L., Zhang, W. Screening and transcriptomic analysis of Crypthecodinium cohnii mutants with high growth and lipid content using the acetyl-CoA carboxylase inhibitor sethoxydim. Applied Microbiology and Biotechnology 2017, 101(15), 6179-6191. (IF=3.376, 二区)
(5)    Sun, T., Chen, L.*, Zhang, W. Quantitative proteomics reveals potential crosstalk between a small RNA CoaR and a two-component regulator Slr1037 in Synechocystis sp. PCC6803. Journal of Proteome Research 2017, 16(8):2954-2963.(IF=4.173, 二区)
(6)    Gao, X., Sun, T., Wu, L., Chen, L.*, Zhang, W. Co-overexpression of response regulator genes slr1037 and sll0039 improves tolerance of Synechocystis sp. PCC 6803 to 1-butanol. Bioresource Technology 2017, doi: 10.1016/j.biortech.2017.04.112.
(7)    王康，刘良森，陈磊*，张卫文。使用稀禾定驯化提高裂殖壶菌的生长和DHA积累。食品与发酵工业 2017, 43(7),42-48.  DOI：10.13995/j.cnki.11-1802/ts.014107
(8)     Pei, G., Niu, X., Zhou, Y., Chen, L.*, Zhang, W. Crosstalk of two-component signal transduction systems in regulating central carbohydrate and energy metabolism during autotrophic and photomixotrophic growth of Synechocystissp. PCC 6803. Integrative Biology 2017, 9(5):485-496. (IF=3.371, 二区)
(9)    陈子熙，陈磊，张卫文。单细胞尺度下的微生物学研究：意义与方法。微生物学报2017,57(6):920-931.
(10)Li, X., Pei, G., Liu, L., Chen, L.*, Zhang, W. Metabolomic analysis and lipid accumulation in a glucose tolerant Crypthecodinium cohnii strain obtained by adaptive laboratory evolution. Bioresource Technology 2017, 235:87-95. (IF=4.917, 一区) （封面文章）
(11)Pei, G., Sun, T., Chen, S., Chen, L., Zhang, W. Systematic and functional identification of small non-coding RNAs associated with exogenous biofuel stress in cyanobacterium Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2017, 10:57. (IF=6.444, 一区)
(12) Shi, M., Zhang, X., Pei, G., Chen, L.*, Zhang, W. Functional diversity of transcriptional regulators in the cyanobacterium Synechocystis sp. PCC 6803. Frontiers in Microbiology 2017, 8:280. (IF=4.165, 二区)
(13) Sun, T., Pei, G., Wang, J., Chen, L.*, Zhang, W.* A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance of Synechocystis sp. PCC6803 to 1-butanol possibly via promoter-directed transcriptional silencing. Biotechnology for Biofuels 2017, 10:42. (IF=6.444, 一区)
(14) 孙韬，陈磊*，张卫文。应用合成生物学策略改良光合蓝细菌。生物产业技术，2017, 01:58-63.
(15) Xie, M., Wang, W., Zhang, W., Chen, L., Lu, X. Versatility of hydrocarbon production in cyanobacteria. Applied Microbiology and Biotechnology 2016, 101(3): 905-919. (IF=3.376, 二区)
(16)Wang, Y., Chen, L.*, Zhang, W*. Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2016, 9:209. (IF=6.444, 一区)
(17) Khan, RI, Wang, Y., Afrin, S., Wang, B., Liu, Y., Zhang, X., Chen, L., Zhang, W., He, L., Ma, G. Transcriptional regulator PrqR plays a negative role in glucose metabolism and oxidative stress acclimation in Synechocystis sp. PCC 6803. Scientific Reports 2016, 6:32507. (IF=5.228, 二区)
(18) Sun, T. Chen, L., Zhang, W. Microbial production of mammalian melatonin - a promising solution to melatonin industry. Biotechnology Journal. 2016, 11(5):601-602. (IF=3.781, 二区)
(19) Chen, S., Zheng, G., Zhu, H., He, H., Chen, L., Zhang, W., Jiang, W., Lu, Y. Roles of two-component system AfsQ1/Q2 in regulating biosynthesis of the yellow-pigmented coelimycin P2 in Streptomyces coelicolor. FEMS Microbiology Letters2016, 363(15). (IF=1.858, 四区)
(20) Xue, Q., Yang, Y., Chen, J., Chen, L., Yang, S., Jiang, W., Gu, Y. Roles of three AbrBs in regulating two-phase Clostridium acetobutylicum fermention. Applied Microbiology and Biotechnology 2016, 100(21):9081-9089. (IF=3.376, 二区)
(21) Fu, Y., Chen, L.*, Zhang, W. Regulatory mechanisms related to biofuel tolerance in producing microbes. Journal of Applied Microbiology 2016, 121(2):320-332. (IF=2.156, 三区)
(22) Zhang, Y., Pei, G., Chen, L.*, Zhang, W.* Metabolic dynamics of Desulfovibrio vulgaris biofilm grown on steel surface. Biofouling, 2016, 32(7):725-736. (IF=3.000, 三区)
(23) Li, T., Zhang, Y., Shi, M., Pei, G. Chen, L.*, Zhang, W.* A putative magnesium transporter Slr1216 involved in sodium tolerance in cyanobacterium Synechocystis sp. PCC 6803. Algal Research, 2016, 17: 202-210. (IF=4.694, 二区)
(24) Zou, X., Pan, T., Chen, L., Tian, Y., Zhang, W. Luminescence materials for pH and oxygen sensing in microbial cells - structures, optical properties, and biological applications. Critical Reviews In Biotechnology 2016, 37(6):723-738. (IF=7.510, 一区)
(25) Qi, Z., Chen, L., Zhang, W. Comparison of Transcriptional Heterogeneity of Eight Genes between Batch Desulfovibrio vulgaris Biofilm and Planktonic Culture at a Single-Cell Level. Frontiers in Microbiology 2016, 7: 597. (IF=4.165, 二区)
(26) Ge, X., Yu, Y., Zhang, M., Chen, L., Chen, W., Elrami, F., Kong, F., Kitten, T., Xu, P. Involvement of NADH Oxidase in Competition and Endocarditis Virulence in Streptococcus sanguinis. Infection and Immunity 2016, 84(5):1470-1477. (IF=3.603, 二区)
(27) Gao, X., Sun, T., Pei, G., Chen, L.*, Zhang, W. Cyanobacterial chassis engineering for enhancing production of biofuels and chemicals. Applied Microbiology and Biotechnology 2016, 100(8), 3401-3413. (IF=3.376, 二区)
(28) Wang,Y., Sun, T., Gao, X., Shi, M., Wu, L., Chen, L., Zhang, W. Biosynthesis of platform chemical 3-hydroxypropionic acid (3-HP) directly from CO2 in cyanobacterium Synechocystis sp. PCC 6803. Metabolic Engineering 2016, 34:60-70. (IF=8.201, 一区)
(29) Wang, J., Chen, L., Chen, Z., Zhang, W. RNA-seq based transcriptomic analysis of single bacterial cells. Integrative Biology, 2015, 7, 1466 - 1476. (IF=3.371, 二区)
(30) Yu, X., Niu, X., Zhang, X., Pei, G., Liu, J., Chen, L.*, Zhang, W. *Identification and mechanism analysis of chemical modulators enhancing astaxanthin acc umulation in Haematococcus pluvialis. Algal Research 2015, 11:284-293. (IF=4.694, 二区)
(31) Gao, L., Pei, G., Chen, L., Zhang, W. A global network-based protocol for functional inference of hypothetical proteins in Synechocystis sp. PCC 6803. Journal of Microbiological Methods 2015, 116:44-52. (IF=1.857, 四区)
(32) Zhang, Y., Niu, X., Shi, M., Pei, G., Zhang, X., Chen, L.*, Zhang, W.* Identification of a transporter Slr0982 involved in ethanol tolerance in cyanobacterium Synechocystis sp. PCC 6803   . Frontiers in Microbiology 2015, 6:487. (IF=4.165, 二区)
(33) Li, J., Niu, X., Pei, G., Sui, X., Zhang, X., Chen, L., Zhang, W. Identification and metabolomic analysis of chemical modulators for lipid accumulation in Crypthecodinium cohnii. Bioresource Technology 2015, 191:362-368. (IF=4.917, 一区)
(34) 徐乐，巫琴，晋虎，陈磊*，张卫文。集胞藻PCC 6803中脂肪酸激活酶Slr1609互作蛋白的鉴定分析。生物工程学报 2015, 31(8): 1194−1202.
(35) 牛向凤，张晓清，于馨恒，苏营雪，陈磊，张卫文。液相质谱联用的代谢组方法优化及其在蓝细菌分析中的应用。生物工程学报 2015，31(4):577-590.
(36) Yu, X., Chen, L., Zhang, W. Chemicals to enhance microalgal growth and accumulation of high-value bioproducts. Frontiers in Microbiology 2015, 6:56. (IF=4.165, 二区)
(37) Niu, X., Zhu, Y., Pei, G., Wu, L., Chen, L.*, Zhang, W.* Elucidating butanol tolerance mediated by a response regulator Sll0039 in Synechocystis sp. PCC 6803 using a metabolomic approach. Applied Microbiology and Biotechnology2015, 99(4):1845-1857. (IF=3.376, 二区)
(38) Zhu, Y., Pei, G., Niu, X., Shi, M., Zhang, M., Chen, L.*, Zhang, W*. Metabolomic analysis reveals functional overlapping of three signal transduction proteins in regulating ethanol tolerance in Synechocystis sp. PCC 6803. Molecular Biosystems 2015, 11(3):770-782. (IF=2.829, 三区)   
(39) Qi, Z., Pei, G., Chen, L., Zhang, W. Single-cell analysis reveals gene-expression heterogeneity in syntrophic dual-culture of Desulfovibrio vulgaris with Methanosarcina barkeri. Scientific Reports 2014, 4:7478. (IF=5.228, 二区)
(40) Sui, X., Niu, X., Shi, M., Li, J., Pei, G., Chen, L., Wang, J., Zhang, W. Metabolomic analysis reveals mechanism of antioxidant butylated hydroxyaniaole on lipid accumulation in Crypthecodinium cohnii.   Journal of Agricultural and Food Chemistry 2014, 62(51):12477-12484. (IF=2.857, 一区)
(41) Pei, G., Chen, L., Wang, J., Qiao, J., Zhang, W. Protein network signatures associated with exogenous biofuels treatments in cyanobacterium Synechocystissp. PCC 6803. Frontiers in Bioengineering and Biotechnology 2014, 2:48.
(42) Wang, Y., Shi, M., Niu, X., Zhang, X., Gao, L., Chen, L., Wang, J. Zhang, W. Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803. Microbial Cell Factories 2014, 13(1):151. (IF=3.744, 二区)
(43) Song, Z., Chen, L.*, Wang, J., Lu, Y., Jiang, W., Zhang, W.* A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803. Molecular & Cellular Proteomics 2014, 13(12):3519-3532. (IF=5.912, 二区)
(44) Su, Y., Wang, J., Shi, M., Niu, X., Yu, X., Gao, L., Zhang, X., Chen, L., Zhang, W. Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions. Bioresource Technology 2014, 170:522-529. (IF=4.917, 一区)
(45) Ren, Q., Shi, M., Chen, L.*, Wang, J., Zhang, W. * Integrated proteomic and metabolomic characterization of a novel two-component response regulator Slr1909 involved in acid tolerance in Synechocystis sp. PCC 6803. Journal of Proteomics 2014, 109C:76-89. (IF=3.867, 二区)
(46) Chen, L., Wu L., Wang, J., Zhang, W. Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2014, 7:89. (IF=6.444, 一区)
(47) Chen, L., Wu, L., Zhu, Y., Song, Z., Wang, J., Zhang, W. An orphan two-component response regulator Slr1588 involves salt tolerance by directly regulating synthesis of compatible solutes in photosynthetic Synechocystis sp. PCC 6803. Molecular Biosystems 2014, 10(7):1765-1774. (IF=2.829, 三区)
(48) Chen, L., Zhu, Y., Song, Z., Wang, J., Zhang, W. An orphan response regulator Sll0649 involved in cadmium tolerance and metal homeostasis in photosyntheticSynechocystis sp. PCC 6803. Journal of Proteomics 2014, 103:87-102. (IF=3.867, 二区)
(49) Wang, J., Zhang, X., Shi, M., Gao, L., Niu, X., Te, R., Chen, L., Zhang, W. Metabolomic analysis of the salt-sensitive mutants reveals changes in amino acid and fatty acid composition important to long-term salt stress in Synechocystissp. PCC 6803. Functional & Integrative Genomics 2014, 14(2):431-440. (IF=2.265, 二区)
(50) Jin, H., Chen, L., Wang, J., Zhang, W. Engineering biofuel tolerance in non-native producing microorganisms. Biotechnology Advances 2014, 32(2):541-548. (IF=9.848, 一区)
(51) Evans, K., Stone, V., Chen, L., Ge, X., Xu, P. Systematic study of genes influencing cellular chain length in Streptococcus sanguinis. Microbiology 2014, 160(Pt 2):307-315. (IF=2.268, 三区)
(52) 任蔷，陈磊，王江新，张卫文。应用合成生物学构建蓝细菌细胞工厂的研究进展。生命科学 2013, 25(10): 952-957。
(53) Wang, J., Chen, L., Tian, X., Gao, L., Niu, X., Shi, M., Zhang, W. Global metabolomic and network analysis of Escherichia coli responses to biofuel stresses. Journal of Proteome Research 2013, 12: 5302−5312. (IF=4.173, 二区)
(54) Zhu, H., Ren, X., Wang, J., Song, Z., Shi, M., Qiao, J., Tian, X., Liu, J., Chen, L.*, Zhang, W.  Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2013, 6(1):106. (IF=6.444, 一区)
(55) 巫琴，陈磊，王江新，张卫文。应用合成生物学策略优化光合蓝细菌底盘。生物工程学报 2013,29(8):1086-1099。
(56) Qiao, J., Huang, S., Te, R., Wang, J., Chen, L., Zhang, W. Integrated proteomic and transcriptomic analysis reveals novel genes and regulatory mechanisms involved in salt stress responses in Synechocystis sp. PCC 6803. Applied Microbiology and Biotechnology 2013, 97(18):8253-8264. (IF=3.376, 二区)
(57) Huang, S., Chen, L., Te, R., Qiao, J., Wang, J., Zhang, W. Complementary iTRAQ proteomics and RNA-seq transcriptomics reveal multiple levels of regulation in response to nitrogen starvation in Synechocystis sp. PCC 6803. Molecular Biosystems 2013, 9(10):2565-2574. (IF=2.829, 三区)
(58) Wang, J., Chen, L., Huang, S., Liu, J., Ren, X., Tian, X., Qiao, J., Zhang, W. RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2012, 5(1):89. (IF=6.444, 一区)
(59) Wang, J., Wu, G., Chen, L., Zhang, W. Cross-species transcriptional network analysis reveals conservation and variation in response to metal stress in cyanobacteria. BMC Genomics 2013, 14:112. (IF=3.867, 二区)
(60) Liu, J., Chen, L., Wang, J., Qiao, J., Zhang, W. Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803. Biotechnology for Biofuels 2012, 5(1):68. (IF=6.444, 一区)
(61) Tian, X., Chen, L., Wang, J., Qiao, J., Zhang, W. Quantitative proteomics reveals dynamic responses of Synechocystis sp. PCC 6803 to next-generation biofuel butanol. Journal of Proteomics 2013, 78:326-345. (IF=3.867, 二区)
(62) Qiao, J., Wang, J., Chen, L., Tian, X., Huang, S., Ren, X., Zhang, W. Quantitative iTRAQ LC-MS/MS Proteomics Reveals Metabolic Responses to Biofuel Ethanol in Cyanobacterial Synechocystis sp. PCC 6803. Journal of Proteome Research 2012, 11(11):5286-5300. (IF=4.173, 二区)
(63) Qiao, J., Chen, L., Li, Y., Wang, J., Zhang, W., Chen, S. Whole-Genome Sequence of Nocardiopsis alba Strain ATCC BAA-2165, Associated with Honeybees. Journal of Bacteriology 2012, 194(22):6358-6359. (IF=3.198, 三区)
(64) Qiao, J., Shao, M., Chen, L., Wang, J., Wu, G., Tian, X., Liu, J., Huang, S., Zhang, W. Systematic characterization of hypothetical proteins in Synechocystis sp. PCC 6803 reveals proteins functionally relevant to stress responses. Gene 2013, 512(1):6-15. (IF=2.319, 四区)
(65) Chen, L., Ge, X., Wang, X., Patel, J.R., Xu, P. SpxA1 involved in hydrogen peroxide production, stress tolerance and endocarditis virulence in Streptococcus sanguinis. PLoS ONE 2012, 7(6): e40034. (IF=3.057, 三区)
(66) Lei, A., Chen, H., Shen, G., Hu, Z., Chen, L., Wang, J. Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors. Biotechnology for Biofuels 2012, 5(1):18. (IF=6.444, 一区)
(67) Xu, P., Ge, X., Chen, L., Wang, X., Dou, Y., Xu, J.Z., Patel, J.R., Stone, V., Trinh, M., Evans, K., Kitten, T., Bonchev, D., Buck, G.A. Genome-wide essential gene identification in Streptococcus sanguinis. Scientific Reports 2011, 1:125. (IF=5.228, 二区)
(68) Chen, L., Ge, X., Dou, Y., Wang, X., Patel, J.R., Xu, P. Identification of hydrogen peroxide production related genes in Streptococcus sanguinis and their functional relationship with pyruvate oxidase. Microbiology 2011, 157:13-20. (IF=2.268, 三区)
(69) Chen, L., Chen, J., Jiang, Y., Zhang, W., Jiang, W., Lu, Y. Transcriptomics analyses reveal global roles of the regulator AveI in Streptomyces avermitilis. FEMS Microbiology Letters 2009, 298(2):199-207. (IF=1.858, 四区)
(70) Wang, W., Shu, D., Chen, L., Jiang, W., Lu, Y. Cross-talk between an orphan response regulator and a noncognate histidine kinase in Streptomyces coelicolor. FEMS Microbiology Letters 2009, 294(2):150-156. (IF=1.858, 四区)
(71) Shu, D., Chen, L., Wang, W., Yu, Z., Ren, C., Zhang, W., Yang, S., Lu, Y., Jiang, W. afsQ1-Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolismg and morphological development in Streptomyces coelicolor. Applied Microbiology and Biotechnology 2009, 81(6):1149-1160. (IF=3.376, 二区)
(72) Chen, L., Lu, Y., Chen, J., Zhang, W., Shu, D., Qin, Z., Yang, S., Jiang, W. Characterization of a negative regulator AveI for avermectin biosynthesis in Streptomyces avermitilis NRRL8165. Applied Microbiology and Biotechnology2008, 80(2):277-286. (IF=3.376, 二区)
(73) Lu, Y., Wang, W., Shu, D., Zhang, W., Chen, L., Qin, Z., Yang, S., Jiang, W. Characterization of a novel two-component regulatory system involved in the regulation of both actinorhodin and a type I polyketide in Streptomyces coelicolor. Applied Microbiology and Biotechnology 2007, 77(3):625-635. (IF=3.376, 二区)
(74) Chen, L., Yu, H., Lu, Y., Jiang, W. Cloning and preliminary characterization of lh3gene encoding a putative acetyltransferase from a rifamycin SV-producing strain Amycolatopsis mediterranei. Biotechnology Letters 2005, 27(15):1129-1134. (IF=1.639, 三区)