中国科学院化学研究所活体分析化学实验室齐莉

dahuangfeng

中山大学化学系，理学学士

河北大学化学与环境科学学院，理学博士

1994-1995年，美国学习

1991年，助研

1997年，副研

2002年-至今，任中科院化学所副研

1992年及1998年分获北京市科技进步奖三等奖及二等奖各一项

2012年获中国分析测试协会科学技术一等奖

兼任北京色谱学会常务理事

中国科学院化学研究所，活体分析化学院重点实验室，北京,100190

Tel: 86-10-82627290 Fax: 86-10-62559373 E-mail: qili@iccas.ac.cn

研究方向        

1. 新型聚合物整体分离介质的合成制备与生物色谱应用研究

2. 基于毛细管电泳技术的手性分析/酶反应动力学研究

3. 微纳聚合物新材料的合成制备与生化应用研究

4. 基于芯片技术的合成与生化分析应用研究

2013年

1.       Juan Qiao, Xiaoyu Mu, Li Qi*, Jingjing Deng, Lanqun Mao. Folic acid-functionalized fluorescent gold nanoclusters with polymer as linker for cancer cells imaging. Chemical Communications, 2013, accepted.

2.       Xiaoyu Mu , Li Qi* , Ping Dong , Juan Qiao, Jian Hou, Zongxiu Nie and Huimin Ma. Facile one-pot synthesis of L-proline-stabilized fluorescent gold nanoclusters and its application as sensing probes for serum iron. Biosensors and Bioelectronics, 2013, 49, 249-255.

3.       Ying Shen, Wei Guo, Li Qi*, Juan Qiao, Fuyi Wang and Lanqun Mao. Immobilization of trypsin via reactive polymer grafting from magnetic nanoparticles for microwave-assisted digestion. Journal of Materials Chemistry B, 2013, 1, 2260-2267.

4.       Guoliang Liu, Daqian Feng, Xiaoyu Mu, Wenjie Zheng, Tianfeng Chen, Li Qi and Dan Li*. DNA-functionalized silver nanoclusters as chemopalette: tunable flurescence for turn-on detection of cysteine. Journal of Materials Chemistry B, 2013, 1, 2128-2131.

5.       Yaping Li, Li Qi*, Huimin Ma. Preparation of porous polymer monolithic column using functionalized graphene oxide as a functional crosslinker for high performance liquid chromatography separation of small molecules. Analyst, 2013, in press.

6.       Juan Qiao, Li Qi*, Huijuan Yan, Yaping Li and Xiaoyu Mu. MCE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor based on gold nanoparticle. Electrophoresis, 2013, 34, 409-416.

7.       Haizhi Zhang, Li Qi*, Ying Shen, Juan Qiao and Lanqun Mao. L-Lysine derived ionic liquids as chiral ligands of Zn(II) complexes used in ligand-exchange capillary electrophoresis. Electrophoresis, 2013, 34, 846-853.

8.       Haizhi Zhang, Li Qi*, Xiaoyu Mu, Xiaoping Zhou, Dan Li and Lanqun Mao. Influence of ionic liquids as electrolyte additives on chiral separation of dansylated amino acids by using Zn(II) complex mediated chiral ligand exchange capillary electrophoresis. Journal of Separation Science, 2013, 36, 886-891.

9.       Jun Yang, Li Qi*, Yi Chen and Huimin Ma. Design and fabrication of a three dimensional spiral micromixer. Chinese Journal of Chemistry, 2013, 31, 209-214.

2012年

1.       Juan Qiao, Li Qi*, Ying Shen, Lingzhi Zhao, Cui Qi, Dihua Shangguan, Lanqun Mao and Yi Chen. Thermal responsive fluorescent block copolymer for intracellular temperature sensing. Journal of Materials Chemistry, 2012, 22, 11543-11549.

2.       Ying Shen, Lingzhi Zhao, Li Qi*, Juan Qiao, Lanqun Mao and Yi Chen. Reactive polymer function as a versatile toolbox for construction of multifunctional superparamagnetic nanocomposites. Chemistry-A European Journal, 2012, 18, 13755-13761.

3.       Xiaoyu Mu, Li Qi*, Ying Shen, Haizhi Zhang, Juan Qiao and Huimin Ma. A novel chiral ligand exchange capillary electrophoresis system with amino acid ionic liquid as ligand and its application in screening D-amino-acid oxidase inhibitors. Analyst, 2012, 137, 4235-4240.

4.       Haizhi Zhang, Li Qi*, Yuqing Lin, Lanqun Mao and Yi Chen. Study on the Decrease of Renal D-Amino Acid Oxidase Activity in the Rat after Renal Ischemia by Chiral Ligand Exchange Capillary Electrophoresis. Amino Acids, 2012, 42, 337-342.

5.       Ying Shen, Li Qi* and Lanqun Mao. Macroporous polymer monoliths with a well-defined three dimensional skeletal morphology. Polymer, 2012, 53, 4128-4139.

6.       Xiaoyu Mu, Li Qi*, Haizhi Zhang, Ying Shen, Juan Qiao and Huimin Ma. Ionic liquids with amino acids as cations: novel chiral ligands in chiral ligand-exchange capillary electrophoresis. Talanta, 2012, 97, 349-354.

7.       Yaping Li, Li Qi*, Juan Qiao, Shen Ying, Huijuan Yan, Peiyong Xin and Huimin Ma. well-defined block copolymer serving as surfactants in separation of 1, 4-dihydropyridines by open tubular capillary eletrochromatography. Electrophoresis, 2012, 33, 2019-2027.

8.       Xiaoyu Mu, Li Qi*, Juan Qiao, Haizhi Zhang and Huimin Ma. Study on glutamic pyruvic transaminase kinetics by microchip electrophoresis. Analytical Biochemistry, 2012, 421, 499-505.

9.       Haizhi Zhang, Li Qi*, Lanqun Mao and Yi Chen. Chiral separation using capillary electromigration techniques based on ligand exchange principle. Journal of Separation Science, 2012, 35, 1236-1248.

10.   Jun Yang, Li Qi*, Huimin Ma and Yi Chen. Fabrication of nylon membrane-based microfluidic chips and its application in color sensing of glucose. Chemical Journal of Chinese Universities, 2012, 11, 2405-2410.

11.   Jun Yang, Li Qi*, Yi Chen and Huimin Ma. A passive mixer with changeable mixing mechanism. Chinese Journal of Chemistry, 2012, 30, 1793-1796.

2011年

1.       Haizhi Zhang, Li Qi*, Juan Qiao and Lanqun Mao. Determination of sodium benzoate by chiral ligand exchange CE based on its inhibitory activity in D-amino acid oxidase mediated oxidation of D-serine. Analytica Chimica Acta, 2011, 691, 103-109.

2.       Chunhe Yao, Li Qi*, Wenbin Hu, Fuyi Wang and Gengliang Yang. Immobilization of trypsin on sub-micron skeletal polymer monolith. Analytica Chimica Acta, 2011, 692, 131-137.

3.       Juan Qiao, Li Qi*, Xiaoyu Mu and Yi Chen. Monolith and coating enzymatic microreactors of L-Asparaginase: kinetics study by MCE-LIF for potential application in ALL treatment. Analyst, 2011, 136(10), 2077-2083.

4.       Ying Shen, Li Qi*, Xiaoyi Wei, Peiyong Xin and Lanqun Mao. Preparation of well-defined environmentally responsive polymer brushes on monolithic surface by two-step atom transfer radical polymerization method for HPLC. Polymer, 2011, 52, 3725-3731.

5.       Ying Shen, Li Qi*, Jianglei Qin, Huijuan Yan, Juan Qiao, Haizhi Zhang, Yongming Chen, Lanqun Mao and Lijun Wan. A novel amphipathic block copolymer coating forming micelle-like aggregates for separation of steroids in open tubular capillary electrochromatography. Talanta, 2011, 84, 501-507.

6.       Peiyong Xin, Ying Shen, Li Qi*, Gengliang Yang and Yi Chen. Preparation of poly(N-isopropylacrylamide)-grafted well-controlled 3D skeletal monolith based on epoxy resin for protein separation. Talanta, 2011, 85, 1180-1186.

7.       Ying Shen, Li Qi*, Jianglei Qin, Juan Qiao, Haizhi Zhang, Yongming Chen and Lanqun Mao. Development of a new open tubular capillary electrochromatography method for in vitro metabolic monitoring of toxic aromatic amines in rat blood. Journal of Separation Science, 2011, 34, 3538-3545.

8.       Jun Yang, Li Qi*, Juan Qiao, Yi Chen and Huimin Ma. Rate acceleration of the Baylis-Hillman Reaction within microreactors. Chinese Journal of Chemistry, 2011, 29, 2385-2388.

2010年

1.       Chunhe Yao, Li Qi*, Juan Qiao, Haizhi Zhang, Fuyi Wang, Gengliang Yang and Yi Chen. High-performance affinity monolith chromatography for enantiomer separation and determination of enzyme kinetic constant. Talanta, 2010, 82, 1331-1336.

2.       Xiaoyi Wei, Li Qi*, Juan Qiao, Chunhe Yao, Fuyi Wang and Yi Chen. Assay of Vitamin B in urine by capillary electrochromatography with methacrylate-based monolithic. Electrophoresis, 2010, 31, 3227-3232.

3.       Li Qi*, Gengliang Yang, Haizhi Zhang and Juan Qiao. A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determination enzyme kinetic constant of L-amino acid oxidase. Talanta, 2010, 81, 1554-1559.

4.       Juan Qiao, Li Qi*, Huimin Ma, Yi Chen, Meixiang Wang and Dexian Wang. Study on amino amides and enzyme kinetics of L- asparaginase by MCE. Electrophoresis, 2010, 31, 1565-1571.

5.       Rongyue Zhang, Li Qi*, Peiyong Xin, Gengliang Yang and Yi Chen. Preparation of macroporous monolith with three dimensional bicontinuous skeleton sturcture by atom transfer radical polymerization for HPLC. Polymer, 2010, 51, 1703-1708.

6.       Xiaoyi Wei, Li Qi*, Junjun Tan, Ruigang Liu and Fuyi Wang. A colorimetric sensor for determination of cysteine by carboxymethyl cellulose-functionalized gold nanoparticles. Analytica Chimica Acta, 2010, 671, 80-84.

7.       Peiyong Xin, Li Qi*, Rongyue Zhang, Chunhe Yao, Xiaoyi Wei, Gengliang Yang and Yi Chen. Well-defined skeletal macroporous polymer monoliths fabricated with a novel type of amphiphilic block copolymer as a phase separator. Polymer, 2010, 51, 3410-3415.

8.       Chunhe Yao, Li Qi*, Gengliang Yang and Fuyi Wang. Preparation of sub-micron skeletal monoliths with high capacity for liquid chromatography. Journal of Separation Science, 2010, 33, 475-483.

2009年

1.       Chunhe Yao, Li Qi*, Hongying Jia, Peiyong Xin, Gengliang Yang* and Yi Chen. A novel glycidyl methacrylate-based monolith with sub-micron skeletons and well-defined macropores. Journal of Materials Chemistry, 2009, 19, 767-772.

2.       Li Qi*, Juan Qiao, Gengliang Yang* and Yi Chen. Chiral ligand-exchange capillary electrophoresis assays for separation of amino acid enantiomers and determination of enzyme kinetic constant. Electrophoresis, 2009, 30, 2266-2272.

3.       Juan Qiao, Li Qi*, Huimin Ma, Huwei Liu, Jun Yang, Yi Chen and Gengliang Yang. A block copolymer covalent coating acting as surfactants in separation of HNE and NBA by capillary electrophoresis. Talanta, 2009, 80, 770-776.

4.       Rongyue Zhang, Gengliang Yang*, Peiyong Xin, Li Qi* and Yi Chen. Preparation of Poly(N-isopropylacrylamide)-Grafted Polymer Monolith for Hydrophobic Interaction Chromatography of Proteins. Journal of Chromatography A, 2009, 1216, 2404-2411.

5.       Xiaoyi Wei, Li Qi*, Gengliang Yang and FuYi Wang. Preparation and characterization of monolithic column by grafting pH-responsive polymer. Talanta, 2009, 79, 739-745.

6.       Li Qi*, Cai Cui, Juan Qiao, Gengliang Yang and Yi Chen. Use of micellar electrokinetic capillary chromatography to analyze reactant and product on Baylis-Hillman reaction. Journal of Separation Science, 2009, 32, 1480-1486.

7.       Juan Qiao, Li Qi*, Huimin Ma and Gengliang Yang. Open tubular CEC with novel block copolymer coatings for separation of aromatic amine. Journal of Separation Science, 2009, 32, 3936-3944.

8.       Rongyue Zhang, Li Qi*, Peiyong Xin, Gengliang Yang and Yi Chen. Preparation and Characterization of Temperature-Responsive Porous Monoliths. Chinese Journal of Chemistry, 2009, 27, 2229-2236.  

sangtian

嵌段聚合物修饰毛细管电色谱柱分离分析解热镇痛药物
冯文雅1,2, 乔娟2,3, 齐莉2,3, 李志伟1
1. 河北科技大学化学与制药工程学院, 石家庄 050018;
2. 中国科学院化学研究所活体分析化学院重点实验室, 北京分子科学国家中心, 北京 100190;
3. 中国科学院大学, 北京 100049


摘要 采用可逆加成断裂链转移聚合法合成了具有两亲性质的嵌段聚合物聚苯乙烯马来酸酐，通过共价键合将其涂覆于毛细管内壁，构建了一种新型开管柱毛细管电色谱（OT-CEC）方法，并将其用于解热镇痛药物的分离分析.考察了缓冲溶液pH值、磷酸盐浓度、有机溶剂的种类及添加量对3种药物（氨基比林、非那西汀和4-氨基安替比林）OT-CEC分离效率的影响.结果表明，在缓冲溶液中添加15%（体积分数）四氢呋喃后，修饰于管壁表面的嵌段聚合物可形成胶束结构，并起到类表面活性剂的作用；与裸管相比，该涂层管可明显提高被分析物的分离效率；连续30次测定电渗流的相对标准偏差<3.0%，表明该涂层管稳定性较好.在最佳OT-CEC分析条件下，对3种药物的分析均获得了良好的定量线性关系（R2>0.988），检出限为3.5~4.8 μg/mL.所建立的方法为新型嵌段聚合物涂层的开发及药物的OT-CEC分析奠定了良好基础.

基金资助:国家自然科学基金（批准号：21727809，21635008，21621062）和中国科学院前沿科学重点研究项目（批准号：QYZDJ-SSW-SLH034）资助.
通讯作者: 李志伟,男,博士,副教授,主要从事药物分析及质量控制方面的研究.E-mail:lizhiwei@hebust.edu.cn;齐莉,女,博士,研究员,主要从事毛细管电泳分析方法方面的研究.E-mail:qili@iccas.ac.cn     E-mail: lizhiwei@hebust.edu.cn;qili@iccas.ac.cn
引用本文:   
冯文雅  乔娟  齐莉  李志伟  . 嵌段聚合物修饰毛细管电色谱柱分离分析解热镇痛药物. 高等学校化学学报, 2018, 39(8): 1640-1646.
FENG Wenya  QIAO Juan  QI Li  LI Zhiwei  . Sepatation of Antipyretic Analgesics by Capillary Electrochromatography with Block Copolymer Coating. Chemical Journal of Chinese Universities, 2018, 39(8): 1640-1646.
链接本文:  
http://www.cjcu.jlu.edu.cn/CN/10.7503/cjcu20170853     或     http://www.cjcu.jlu.edu.cn/CN/Y2018/V39/I8/1640