王家海,广州大学教授,University of Florida分析化学博士学位,Johns Hopkins University 化学生物工程系博士后,广州大学化学化工学院教授学术带头人。团队目前在在国际期刊JACS,Advanced Materials ,Advanced Functional Materials,Nano letter,Biosensor and Bioelectronics,Nanoscale,Analytical Chemistry,ACS. Appl. Mater. Interface,上发表系列文章。单篇入选2016年中国百篇最具影响国际学术论文。
一基本情况 姓名:王家海 出生年月:1978/06/18 职务:广州大学百人计划教授学术带头人 部门:广州大学化学化工学院 http://nanopore.weebly.com/publication-list.html 二教育背景和工作经历 1996.09-2000.07 湖北大学 化学系 学士学位。 2000.08-2003.08 中科院大连化学物理研究所 物理化学 硕士学位 导师:何国钟院士/韩克利研究员 2003.08-2008.05 University of Florida分析化学 博士学位 导师:Charles Ray. Martin 2008.05-2008.12 Johns Hopkins University 化学生物工程系 博士后 导师:David Gracias 2009.01-2009.05 中科院苏州纳米所 国际纳米实验室 副研究员 2009.08-2014.08 中科院长春应用化学研究所 电分析国家重点实验室 副研究员 2014.10-2017.11 山东大学 国家胶体材料工程技术研究中心 研究员 2017.03-现在 广州大学 化学化工学院 百人计划教授 学术带头人 三科学贡献和成果介绍 本人主要在核酸探针和仿生纳米孔两方面开展了一系列分子识别的工作,也为将来进一步开展分析化学研究打下了坚实的基础,期间积累了多种前沿分析方法和技术:仿生纳米孔制备和检测;微纳米加工技术;核酸探针人工合成技术。这两个子方向相互融合和相互促进。 核酸四极子(G-quadruplex)是一种具有特殊结构的核酸分子探针,核酸四极子能够稳定平面分子(发微弱荧光),提高小分子荧光强度。本人及其团队成员,与瑞士科学家合作,首次合成小分子tetrakis-(diisopropylguanidino) zinc phthalocyanine (Zn-DIGP)并结合G-quadruplex高选择性检测了血液中的钾离子,对钾离子的检测区间可根据需要进行调控,在极高钠离子浓度(3500倍高于钾离子)存在的情况下,效检测微摩尔钾离子(Analytical Chemistry,2010.82(19):p. 8356-8360.)。本人申请并搭建了一整套合成,纯化核酸的系统。我们进一步扩展G-quadruplex的应用,和小分子N-methyl mesoporphyrin IX(NMM)结合高效检测了铜离子(Chemical Communications,2010.46(39):p. 7385-7387.)。除了能探测离子外,特定序列的G-quadruplex能选择性探测小分子毒素(OTA),结合石墨烯后,灵敏度更高,检测限更低,引用率一百多次(Biosensors and Bioelectronics,2011.26(8):p. 3494-3499.)。完整的G-quadruplex也可以被对称和非对称劈开,我们首次在国际上用劈开式探针和荧光小分子选择性检测病毒基因,并详细解释了检测机理(Biosensors and Bioelectronics,2012.31(1): p.316-322.)。基于G-quadruplex,我们构建了免标记的荧光信号放大体系,可以用于检测各种低浓度的目标对象(Chemical Communications,2011.47(38):p. 10563-10565.)。针对手型多肽的检测,利用功能核酸探针,发展和设计了荧光,电化学,比色法等多种手段检测多肽分子,极大地丰富了针对手型多肽的检测方法。 核酸探针除了能在溶液中检测目标对象,修饰在固态电极和纳米材料表面能更进一步发挥其作用。我们设计了一种在电极表面修饰核酸探针的新方法(Insertion approach)(Analytical Chemistry,2014. 86(10), 4657-4662.)。过去十多年,在电极表面修饰核酸探针是先修饰探针,后填充小分子(MCH)消除非特异性吸附,其缺点表现为重现性差,稳定性差,失败率超高。我们新设计的这种表面方法是先修饰小分子(MCH),然后修饰核酸探针(插入小分子之间的空隙),与信号放大体系结合能进一步体现其优势,极大地提高了信号重现性,探针在电极表面的稳定性。 经过20多年的发展,纳米孔分析化学取得了巨大的进步。申请者本人(A)为了提高纳米孔在分析化学上的应用范围和深度,在国际上首次把离子整流原理用于纳米孔分析化学,分析药物分子,两次在国际大会专题报告(Nanomedicine, 2008.3(1): p. 13-20.)。(B)发明了光透射技术测量纳米孔孔径(Chemical Communications,2013.49(97): p.11415-11417.)。(C)借助纳米通道支撑基底,发现高分子膜材料上具备完美的离子二极管效应和离子整流现象,离子整流系数达几万倍(RSC Adv., 2015,5, 35622-35630.),将进一步用在分析化学上。(D)限域纳米孔内的立体阻碍干扰探针和探测物的相互作用,为了消除这个干扰,设计了无探针修饰的纳米孔分析平台(Biosensors and Bioelectronics,2015, 63(15):287-293.)。(E)运用离子整流解释了高分子薄膜内羧基可以带正电,打破传统观点(Nanoscale,2011.3(9): p. 3767-3773.)。(F)把二维的纳米孔折叠成三维的微米器件,运用于细胞培养和药物释放。 2016年尝试了新的研究方向,合成纳米线阵列,除了运用在电分解水,制备洁净能源的制备上(Adv. Mater., 2016,28, 215-230.),我们将进一步将其运用在生命分析和环境分析。 四文章列表(*通讯作者) 1. Shili Qin, Jinlong Lei, Yun Xiong,Xiaohu Xu, Xinhua Geng,Jiahai Wang*,Synthesis of Ni4.5Fe4.5S8/Ni3S2filmon Ni3Fe alloy foam as an excellent electrocatalyst for the oxygenevolution reaction,RSC Adv.,2019,9,10231-10236 2. Wei Yang, Bin Tian, Yuguo Xia, FenggangLiu, Shujie Chen, Xinfa Dong, Shengzhou Chen,Jiahai Wang*,Engineeringmultiphase for activating electroactive sites for highly efficient hydrogenevolution: Experimental and theoretical investigation,Int. J. HydrogenEnergy, 2019,44,26, 13323-13333 3. Qinghe Cao, Chuanqin Wang, Shujie Chen,Xingdong Xu, Fenggang Liu, Xinhua Geng,Jiahai Wang*,Vertically alignedNiP2 nanosheets with interlaced mesh network for highly efficient watersplitting under alkaline and acid solutions,Int. J. Hydrogen Energy,2019,44, 13, 6535-6543 4. Shujie Chen, Jinhui Zhang, MingfengYang, Fenggang Liu, Zhipeng Xie, Yunlin Liu, Wenxia Lin, Daru Wang, XiangruiLi,Jiahai Wang*,Diastereoselective synthesis of cyclopropanes bearingtrifluoromethyl-substituted all-carbon quaternary centers from2-trifluoromethyl-1,3-enynes beyond fluorine elimination,Chem. Commun,2019,55,3879-3882 5. Huajun Xu, Fenggang Liu, Shujie Chen,Wenjing Shi, Zhipeng Xie, Jianwu Wang, Zhaofen Zhai, Jianpeng Liu, Han Li,JiahaiWang*,Design and synthesis of novel H-Shaped chromophore for enhancednonlinear optical properties,Dyes Pigments,2019, 165, 144-150 6.FenggangLiu, Hua zhang, Hongyan Xiao, Huajun Xu, Shuhui Bo, Ling Qiu, Zhen zhen, LijieLai, Shujie Chen,Jiahai Wang*,Dyes Pigments,2018 Shengzhou Chen, QiuchanHuang, Wei Yang, Hanbo Zou, Huangwang Mai,JiaHai Wang*,Pt-CoNsupported on TiN-modified carbon nanotubes (Pt-CoN/TiN–CNT) asefficient oxygen reduction reaction catalysts in acidic medium,Int. J. Hydrogen Energy,2018,43, 31, 14337-14346 8. Zhipeng Xie, Lei Jinglong, YangMingfeng, Youji Li, Xinhua Geng, Simin Liu,Jiahai Wang*,Conicalnanofluidic channel for selective quantitation of melamine in combination with β-cyclodextrinand a single-walled carbon nanotube,Biosensors and Bioelectronics,2018,127,200-206 9. Bin Tian, Jingrong Lei,Jiahai Wang*,Polyoxometalate–SurfactantHybrids Directed Assembly of Ni3S2 into Hollow Microsphere as Pt-ComparableElectrocatalyst for Hydrogen Evolution Reaction in Alkaline Medium,ACS.Appl. Mater. Interface,2017,9(46), pp 40162–40170. 10. Wang, C.; Zhang, P.; Lei, J.; Dong,W.*; Wang, Jiahai*., Integrated 3D MoSe2@Ni0.85Se Nanowire Network withSynergistic Cooperation as Highly Efficient Electrocatalysts for HydrogenEvolution Reaction in Alkaline Medium.Electrochimica Acta2017, 246(Supplement C), 712-719. 11. Wu, M.; Wang, S.*;Wang, Jiahai*,Engineering NiMo3S4|Ni3S2 interface for excellent hydrogen evolution reactionin alkaline medium.Electrochimica Acta2017, 10.1016/j.electacta.2017.11.112. 12. Chuanqin Wang, Bin Tian, Mei Wu,JiahaiWang*, Revelation of its excellent intrinsic activity of MoS2|NiS|MoO3nanowirearrays for hydrogen evolution reaction in alkaline medium,ACS. Appl. Mater.Interface, 2017, 9, 7084-7090.(IF 7.15) 13.Jiahai Wang, Wei Cui, Qian Liu,Zhicai Xing, Abdullah M. Asiri, Xuping Sun*, Recent progress in Co-basedheterogeneous catalysts for electrochemical water splitting,Adv. Mater.,2016,28, 215-230. (IF 18.96)入选中国科技论文统计结果中2016年中国百篇最具影响国际学术论文) 14. Guo, Z.,Jiahai. Wang*, J. Ren,and E. Wang*, pH-Reversed ionic current rectification displayed by conicallyshaped nanochannel without any modification.Nanoscale,2011.3(9):p. 3767-3773.(IF 7.76). 15. Siqi Zhang, Amin Bao, Ting Sun*, ErkangWang*,Jiahai Wang*,PEI/Zr4+-coated nanopore for selective and sensitivedetection of ATP in combination with single-walled carbon nanotubes,Biosensorsand Bioelectronics,2015, 63(15):287-293. (IF 7.47) 16. Ren, J.,Jiahai. Wang*, J. Wang*,N.W. Luedtke, and E. Wang*,Enantioselective and label-free detection of oligopeptidevia fluorescent indicator displacement.Biosensors and Bioelectronics,2012.35(1):p. 401-406.(IF 7.47) 17. Ren, J.,Jiahai. Wang*, J. Wang,N.W. Luedtke, and E. Wang*,Contribution of potassium ion and split modes ofG-quadruplex to the sensitivity and selectivity of label-free sensor toward DNAdetection using fluorescence.Biosensors and Bioelectronics,2012.31(1):p. 316-322.(IF 7.47) 18. Sheng, L., J. Ren, Y. Miao,Jiahai.Wang*, and E. Wang*,PVP-coated graphene oxide for selective determinationof ochratoxin A via quenching fluorescence of free aptamer.Biosensors andBioelectronics,2011.26(8): p. 3494-3499.(IF 7.47;Citation:>100) 19. Qingfeng Zhai, Siqi Zhang, Hong Jiang,Qin Wei*, Erkang Wang* andJiahai Wang*, Biomimetic nanopore forsensitive and selective detection of Hg(II) in conjunction with single-walledcarbon nanotubes,J. Mater. Chem. B, 2014,2, 6371-6377. (IF: 4.87) 20. Hou, S.,Jiahai. Wang, and C.R.Martin,Template-synthesized DNA nanotubes.Journal of the American ChemicalSociety,2005.127(24): p. 8586-8587.(IF 13.08) 21. Ren, J.,Jiahai. Wang,J. Wang*,and E. Wang*,Inhibition of G-quadruplex assembling by DNA ligation: A versatileand non-covalent labeling strategy for bioanalysis.Biosensors andBioelectronics,2014.51: p. 336-342. (IF 7.47) 22. Hou, S.,Jiahai. Wang, and C.R.Martin,Template-synthesized protein nanotubes.Nano Letters,2005.5(2):p. 231-234.(IF 13.78) 23. Yang, L., C. Zhai, H. Jiang, G. Li*,Jiahai.Wang*, and E. Wang*, Insertion Approach: Bolstering the Reproducibility ofElectrochemical Signal Amplification via DNA Superstructures.AnalyticalChemistry,2014. 86(10), 4657-4662.(IF:5. 89) 24. Qin, H., J. Ren,Jiahai. Wang*,N.W. Luedtke, and E. Wang*,G-quadruplex-modulated fluorescence detection ofpotassium in the presence of a 3500-fold excess of sodium ions.AnalyticalChemistry,2010.82(19): p. 8356-8360.(IF:5. 89) 25. Yang, L., Q. Zhai, G. Li, H. Jiang, L.Han,Jiahai. Wang*, and E. Wang*,A light transmission technique for poresize measurement in track-etched membranes.Chemical Communications,2013.49(97):p. 11415-11417.(IF:6.57) 26. Ren, J.,Jiahai. Wang*, L. Han,E. Wang*, and J. Wang*,Kinetically grafting G-quadruplexes onto DNA nanostructuresfor structure and function encoding via a DNA machine.ChemicalCommunications,2011.47(38): p. 10563-10565.(IF:6.57) 27. Qin, H., J. Ren,Jiahai. Wang*,and E. Wang*,G-quadruplex facilitated turn-off fluorescent chemosensor forselective detection of cupric ion.Chemical Communications,2010.46(39):p. 7385-7387.(IF:6.57) 28.Wang, Jiahai.and C.R. Martin,Anew drug-sensing paradigm based on ion-current rectification in a conicallyshaped nanopore.Nanomedicine,2008.3(1): p. 13-20.(IF:4.99) 29. Qin, H., J. Liu, C. Chen,Jiahai.Wang*, and E. Wang*,An electrochemical aptasensor for chiral peptidedetection using layer-by-layer assembly of polyelectrolyte-methyleneblue/polyelectrolyte-graphene multilayer.Analytica chimica acta,2012.712:p. 127-131.(IF:4.72) 30. Ren, J.,Jiahai. Wang, J. Wang*,and E. Wang*,Colorimetric enantiorecognition of oligopeptide and logic gateconstruction based on DNA aptamer–ligand–gold nanoparticle interactions.Chemistry-A European Journal,2013.19(2):p. 479-483.(IF:5.78) 31. Siqi Zhang, Ting Sun,Jiahai Wang*,Biomimeticphosphate assay based on nanopores obtained by immobilization of Zirconium(IV)on a film of polyethyleneeimine,Microchimica acta,2015,59(35):4946-4952. (Impact factor:4.87) 32. Guo, Z.,Jiahai. Wang*, and E.Wang*,Selective discrimination of small hydrophobic biomolecules based onion-current rectification in conically shaped nanochannel.Talanta,2012.89:p. 253-257.(Impact factor:4.03) 33. Qingfeng Zhai,Jiahai Wang*, HongJiang*, Qin Wei*, Erkang Wang, Bare conical nanopore embedded in polymermembrane for Cr(III) sensing,Talanta, 2015,140, 219-225(Impactfactor:4.03) 34. Guo, Z., J. Ren,Jiahai. Wang*,and E. Wang*,Single-walled carbon nanotubes based quenching of free FAM-aptamerfor selective determination of ochratoxin A.Talanta,2011.85(5):p. 2517-2521.(Impact factor:4.03) 35. Hong Jiang, Erkang Wang,Jiahai Wang*,Gas-breathing polymer for constructing of switchable ionic diode,RSC Adv.,2015,5, 35622-35630.(Impact factor:3.29) 36. Guo, Z.,Jiahai. Wang*, and E.Wang*,Signal-amplification detection of small molecules by use ofMg2+-dependent DNAzyme.Analytical and bioanalytical chemistry,2013:p. 1-7.(IF:3.12) 37. Ren, J., H. Qin,Jiahai. Wang*,N.W. Luedtke, E. Wang*, and J. Wang*,Label-free detection of nucleic acids byturn-on and turn-off G-quadruplex-mediated fluorescence.Analytical andbioanalytical chemistry,2011.399(8): p. 2763-2770.(IF:3.12) 38. Lv, L., Z. Guo,Jiahai. Wang*,and E. Wang*,G-quadruplex as signal transducer for biorecognition events.Currentpharmaceutical design, Invited Review,2012.18(14): p.2076-2095.(IF:3.05) 39.Wang, Jiahai, K.-L. Han, G.-Z.He, Z. Li, and V.R. Morris,Theoretical Study of the C2(1Σg+, 3Πu)+ H2OReaction Mechanism.The Journal of Physical Chemistry A,2003.107(46):p. 9825-9833.(IF:2.88) 40. Siqi Zhang, Ting Sun, Erkang Wang,JiahaiWang*,Investigation of self-assembled protein dimers through an artificalion channel for DNA Sensing,Chinese Science Bulletin, 2014,59(35):4946-4952. (IF: 1.789) 41. Li, Z., P. Nguyen, M. Fatima de Leon,JiaHai.Wang,K. Han, and G.Z. He,Experimental and theoretical study of reaction ofOH with 1, 3-butadiene.The Journal of Physical Chemistry A,2006.110(8):p. 2698-2708.(IF:2.88) 42.Wang, Jiahai, K.-L. Han, G.-Z.He, and Z. Li,Ab initio study of reaction mechanism of C2+H2S.ChemicalPhysics Letters,2003.368(1): p. 139-146.(IF: 1.86) 43.Wang, Jiahai., M. Patel, and D.H.Gracias,Self-assembly of three-dimensional nanoporous containers.Nano,2009.4(01):p. 1-5.(IF: 0.95) 44. Zhijun, G.,Wang. Jiahai*, H.Yaohui*, and W. Erkang*,Application of Biomimetic Nanopore Fabricated inSelf-Supported Membrane in Analytical Chemistry.Progress in Chemistry,2011.23(10):p. 2103-2112.(IF:0.894) 博士毕业题目: 1.Wang, Jiahai.,Fabrication ofRobust Biomimetic Nanopore for Biomolecular and Biomedical Analysis.2008,University of Florida. 五项目申请 序号项目名称 项目来源 起止时间 第一单位 负责人 经费(万元) 本人角色 1 长春应化所人才引进专项 长春应化所2009.08-2013.12长春应化所 王家海30万 项目负责人 2 自组装的单纳米孔与单纳米粒子器件对重金属离子的快速检测No.20905056,国家基金委青年科学基金,2010.01-2012.12,长春应化所,王家海,19万,项目负责人 3 与微流控集成的锥形纳米孔在电生命分析化学中的应用No. 21275137国家基金委面上科学基金2013.01-2016.12长春应化所王家海80万项目负责人 4 运用小分子探针与纳米孔区分核酸双链中碱基突变No. 21575078国家基金委面上科学基金2016.01-2019.12山东大学王家海65万(直接经费)项目负责人 5 中科院创新促进会资助经费 中科院2011.05-2014.12长春应化所 王家海40万 项目负责人 6 国家重点实验室支持项目 重点实验室 每年 长春应化所 王家海5万 项目负责人 7 重点实验室仪器专项 财政部2010.10-2011.12长春应化所 王家海100万 项目负责人,平台搭建人 8 光控适配子的智能化设计,合成及其应用No.21075120国家基金委面上科学基金2011.01-2013.12长春应化所汪尔康35万,第一骨干(王家海) 六学术报告和交流 ●Wang, JiaHai; Choi, Youngseon; Hou,ShiFeng; Martin, C. R. “DNA functionalized nanotube membranes for facilitated transport ofcomplementary DNA,” Abstracts of papers,231st ACS National Meeting, Altanta, GA,United States, March 26-30, 2006. ●Wang, JiaHai;John Wharton, Pu Jin;Martin, C. R. “Small molecule responsive conical nanotube embedded in polymermembrane,”PITTCON2008, New Orleans, LA, UnitesStates, March 2-6, 2008. ●王家海,“Fabrication of robust bio-mimetic nanoporous materials for biosensorand biomedical device.”中科院苏州纳米所,2008年8月27日,主持人:国际实验室王强斌研究员。 ●王家海,“新一代单纳米孔和多纳米孔器件在生物传感器和医学工程中的应用.”中科院大连化学物理研究所,2009年2月17日,主持人:分子反应国家重点实验室韩克利研究员。 ●王家海,“一维和三维纳米孔器件在生物传感器和细胞治疗中的应用.”浙江大学,2009年3月7日,主持人:聚合反应工程国家重点实验室王立教授。 ●王家海,“一维和三维纳米孔器件在生物传感和疾病治疗中的应用.”厦门大学,2009年4月3日,主持人:固体表面物理化学国家重点实验室杨朝勇教授。 ●王家海,“一维和三维纳米孔器件在生物传感和疾病治疗中的应用.”湖南大学,2009年4月15日,主持人:化学生物传感与计量学国家实验室主任吴海龙教授。 ●王家海,“一维和三维纳米孔器件在生物传感和疾病治疗中的应用.”武汉大学化学院,2009年5月17日,主持人:黄卫华教授。 ●王家海,“一维和三维纳米孔器件在生物传感和疾病治疗中的应用.”中科院长春应用化学研究所,2009年6月15日,主持人:电分析国家重点实验室汪尔康院士。 ●王家海,“仿生单纳米通道和四极子核酸探针在分析化学中的应用”.湖北大学有机功能分子合成与应用教育部重点实验室,2011年04月17日,主持人:王升富教授。 ●Jiahai Wang,“pH-reversed ion current rectification via conically-shaped nanoporewithout any modification”, Feburary 14,2012,Nanopore conference. Spain. ●王家海,“三维智能化仿生材料和器件在分析化学中的应用”2012年04月15日,第28届化学会,成都。 ●王家海,“Conically-shaped nanofluidics for analytical chemistry”2012年05月23日,AMN2012@DICP,大连。 ●王家海,“光透射技术精确测量纳米孔”2014年04月12日,第12届全国电分析化学会,桂林。
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