浙江大学化学工程与生物工程学院叶丽丹

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叶丽丹，博士，浙江大学化学工程与生物工程学院副教授，博士生导师。2004年在浙江大学获得理学学士学位，2006年在浙江大学获得理学硕士学位，2010年在德国耶拿大学获得生物学博士学位, 2010-2013年在新加坡国立大学和新加坡科技研究局从事博士后研究，2013年起在浙江大学工作。主要研究方向是微生物合成生物学，利用代谢工程和蛋白质工程等手段探讨微生物合成过程中的关键问题，已在Metabolic Engineering, Biotechnology and Bioengineering, ACS Synthetic Biology等生物工程领域国际知名刊物发表第一作者/通讯作者SCI论文20余篇。

姓名：叶丽丹

单位：化学工程与生物工程学院

职称：副教授

个人简介

学习经历：

2006.9-2010.3 德国耶拿大学生物与药学学院 理学博士

2004.9-2006.6 浙江大学生命科学学院 理学硕士

2000.9-2004.6 浙江大学生命科学学院 理学学士

工作经历：

2015.1-至今     浙江大学化学工程与生物工程学院  副教授

2013.8-2014.12 浙江大学化学工程与生物工程学院 讲师

2013.2-2013.8   新加坡科技研究局化学与工程科学研究院 Scientist

2010.8-2013.2   新加坡国立大学化学与生物分子工程系 博士后研究员

工作研究领域

（1）微生物代谢工程；（2）合成生物学技术。

联系方式

电子信箱：yelidan@zju.edu.cn

2018

1.        PingpingZhou, Wenping Xie, Zhen Yao, Yongqiang Zhu, Lidan Ye, Hongwei Yu.Development of a temperature-responsive yeast cell factory using engineeredGal4 as a protein switch. Biotechnology and Bioengineering, 2018,DOI: 10.1002/bit.26544

2.        LidanYe, Chengcheng Yang, Hongwei Yu*. From molecular engineering to processengineering: Development of high-throughput screening methods in enzymedirected evolution. Applied Microbiology and Biotechnology, 2018,102(2):559-567

2017

1.        PingpingZhou, Wenping Xie, Aipeng Li, Fan Wang, Zhen Yao, Qi Bian, Yongqiang Zhu,Hongwei Yu, Lidan Ye*. Alleviation of metabolic bottleneck bycombinatorial engineering enhanced astaxanthin synthesis in saccharomycescerevisiae. Enzyme and Microbial Technology, 2017, 100: 28-36.

2.        FanWang, Xiaomei Lv, Wenping Xie, Pingping Zhou, Yongqiang Zhu, Zhen Yao,Chengcheng Yang, Xiaohong Yang, Lidan Ye*, Hongwei Yu* CombiningGal4p-mediated expression enhancement and directed evolution of isoprenesynthase to improve isoprene production in Saccharomyces cerevisiae, MetabolicEngineering, 2017, 39:257-266.

3.        LidanYe1, Xiaomei Lv1, Hongwei Yu*Assembly of BiosyntheticPathways in Saccharomyces cerevisiae Using A Ready-to-use Marker RecyclableIntegrative Plasmid Toolbox, Frontiers of Chemical Science andEngineering, 2017, 11(1): 126-132

4.        YongqiangZhu, Wenqiang Lu, Lidan Ye, Zhaofeng Chen, Weijiang Hu, JianboChen, Hongwei Yu*. Enhanced synthesis of Coenzyme Q 10, by reducing thecompetitive production of carotenoids in Rhodobacter sphaeroides. BiochemicalEngineering Journal, 2017, 125:50-55.

5.        YongqiangZhu, Lidan Ye, Zhaofeng Chen, Weijiang Hu, Yanghui Shi, Jianbo Chen, Chenfei Wang, Yong Li, Weifeng Li, Hongwei Yu*. SynergicRegulation of Redox Potential and Oxygen Uptake to Enhance Production ofCoenzyme Q 10, in Rhodobacter sphaeroides. Enzyme &Microbial Technology, 2017, 101, 36-43

6.        XiaohongYang, Lidan Ye, Aipeng Li, Chengcheng Yang, Huilei Yu, Jiali Gu,Fei Guo, Ling Jiang, Fan Wang, Hongwei Yu* Engineering ofD-Fructose-6-Phosphate Aldolase A for Improved Activity towardsCinnamaldehyde, Catalysis Science & Technology, 2017, 7:382-386.

7.        ChengchengYang, Lidan Ye, Jiali Gu, Xiaohong Yang, Aipeng Li, Hongwei Yu*Directed evolution of mandelate racemase by a novel high-throughput screeningmethod, Applied Microbiology and Biotechnology, 2016,101(3):1063-1072.

2016

1.        LidanYe, Xiaomei Lv, Hongwei Yu* Engineering microbes for isopreneproduction. Metabolic Engineering, 2016, 38:125-138.

2.        XiaomeiLv, Jiali Gu, Fan Wang, Wenping Xie,Min Liu, Lidan Ye*, Hongwei Yu*Combinatorial pathway optimization in Escherichia coli by directed co-evolutionof rate-limiting enzymes and modular pathway engineering, Biotechnologyand Bioengineering, 2016, 113(12):2661-2669.

3.        WeiweiChen, Hongwei Yu, Lidan Ye* Comparative study on differentexpression hosts for alkaline phytase engineered in Escherichia coli, AppliedBiochemistry and Biotechnology, 2016, 179(6):997-1010.

4.        GuoFei#, Ye Lidan#, Li Aipeng, Yang Xiaohong,Yang Chengcheng, Yu Hongwei* Insight into the Role of HalogenBond in the Activity of D-Mandelate Dehydrogenase toward HalogenatedSubstrates, Tetrahedron Letters, 2016, 57(18): 1944-1948.

5.        XiaomeiLv, Fan Wang, Pingping Zhou, Lidan Ye, Wenping Xie, Haoming Xu& Hongwei Yu* Dual regulation of cytoplasmic and mitochondrial acetyl-CoAutilization for improved isoprene production in Saccharomycescerevisiae, Nature Communications, 2016, 7, 12851.

6.        AipengLi, Lidan Ye, Xiaohong Yang, Bei Wang, Chengcheng Yang, Jiali Gu, HongweiYu* Reconstruction of the catalytic pocket and enzyme-substrate interactionsenhanced the catalytic efficiency of a short-chaindehydrogenase/reductase, ChemCatChem, 2016, DOI:10.1002/cctc.201600921.

7.        AipengLi, Lidan Ye, Xiaohong Yang, Chengcheng Yang, Jiali Gu, Yu Hongwei*Structure-guided stereoselectivity inversion of a short-chaindehydrogenase/reductase towards halogenated acetophenones, ChemicalCommunications, 2016, 52(37): 6284-6287.

8.        YanLyu, Lidan Ye, Jun Xu, Xiaohong Yang, Weiwei Chen, Hongwei Yu*.Recent research progress with phospholipase C from Bacillus cereus. BiotechnologyLetters, 2016, 38(1):23-31.

2015

1.        LidanYe, Wenping Xie, Pingping Zhou, Hongwei Yu*. Biotechnological Production ofAstaxanthin through Metabolic Engineering of Yeasts, ChemBioEng Reviews,2015, 2(2): 107-117

2.        AipengLi, Lidan Ye, Hongping Wu, Xiaohong Yang, Hongwei Yu*Characterization of an excellent anti-Prelog short-chaindehydrogenase/reductase EbSDR8 from Empedobacter brevis ZJUY-1401, Journalof Molecular Catalysis B: Enzymatic, 2015, 117:31-37.

3.        PingpingZhou, Lidan Ye, Wenping Xie, Xiaomei Lv, Hongwei Yu* Highlyefficient biosynthesis of astaxanthin in Saccharomyces cerevisiae byintegration and tuning of algal crtZ and bkt, Applied Microbiology andBiotechnology, 2015, 99(20): 8419-8428.

4.        JialiGu, Hongfei Tong; Lidan Ye, Hongwei Yu* The role of aromaticresidue W20 in the activity and enantioselectivity control of esterase BioHtowards aryl substrate, Biochemical Engineering Journal, 2015, 101:134-141.

5.        AipengLi, Lidan Ye, Fei Guo, Xiaohong Yang, Hongwei Yu * Biocatalyticanti-Prelog reduction of prochiral ketones with whole cells of a newly isolatedstrain Empedobacter brevis ZJUY-1401, Journal of Molecular Catalysis B:Enzymatic, 2015, 117,31-37.

6.        WenpingXie, Xiaomei Lv, Lidan Ye, Pingping Zhou, Hongwei Yu* Constructionof lycopene-overproducing Saccharomyces cerevisiae by combining directedevolution and metabolic engineering, Metabolic Engineering, 2015,30: 69-78.

7.        WenqiangLu, Lidan Ye, Xiaomei Lv, Wenping Xie, Jiali Gu, Zhaofeng Chen, YongqiangZhu, Aipeng Li, Hongwei Yu*, Identification and elimination of metabolicbottlenecks in the quinone modification pathway for enhanced coenzyme Q10production in Rhodobacter sphaeroides, Metabolic Engineering,2015, 29: 208–216.

8.        WeiweiChen, Lidan Ye, Fei Guo, Yan Lv, Yu Hongwei*, Enhancedactivity of an alkaline phytase from Bacillus subtilis 168 inacidic and neutral environments by directed evolution, BiochemicalEngineering Journal, 2015, 98: 137-143.

9.        JialiGu, Lidan Ye, Fei Guo, Xiaomei Lv, WenqiangLu, Hongwei Yu*.Improved apparent enantioselectivity of a hydrolase by sequential hydrolysisand racemization, Tetrahedron Letters, 2015, 56 (12):1489–1491.

10.    Wenping Xie, LidanYe, Xiaomei Lv, Haoming Xu, Hongwei Yu*. Sequential control of biosyntheticpathways for balanced utilization of metabolic intermediates in Saccharomycescerevisiae. Metabolic Engineering, 2015, 28:8-18.

11.    Jiali Gu, LidanYe,Fei Guo, Xiaomei Lv, Wenqiang Lu, Hongwei Yu*. Rational design ofesterase BioH with enhanced enantioselectivity towards methyl(S)-o-chloromandelate. Applied Microbiology and Biotechnology, ,2015, 99:1709–1718.

2014

1.        XiaomeiLv, Wenping Xie, Wenqiang Lu, Fei Guo, Jiali Gu, Hongwei Yu, Lidan Ye*Enhanced isoprene biosynthesis in Saccharomyces cerevisiae by engineering ofthe native acetyl-CoA and mevalonic acid pathways with a push-pull-restrainstrategy. Journal of Biotechnology, 2014, 186: 128–136.

2.        MinLiu, Wenping Xie, Haoming Xu, Jiali Gu, Xiaomei Lv, Hongwei Yu, LidanYe*. Directed evolution of an exoglucanase facilitated by a co-expressedβ-glucosidase and construction of a whole engineered cellulase system in Escherichiacoli. Biotechnology Letters, 2014, 36:1801–1807.

3.        LingJiang, Bo Wang, Rongrong Li, Sasa Shen, Hongwei Yu, Lidan Ye*.Catalytic promiscuity of Escherichia coli BioH esterase:application in the synthesis of 3,4-dihydropyran derivatives. ProcessBiochemistry, 2014, 49(7):1135–1138.

4.        LingJiang, Bo Wang, Rongrong Li, Sa Shen, Hongwei Yu, Lidan Ye*.“Amano” Lipase DF-catalyzed efficient synthesis of 2,2’-arylmethylenedicyclohexane-1,3-dione derivatives in anhydrous media. ChineseChemical Letters, 2014, 25(8):1190-1192.

5.        LidanYe, Mohammad Sufian Bin Hudari, Zhi Li*, Jin Chuan Wu*. Simultaneousdetoxification, saccharification and co-fermentation of oil palm empty fruitbunch hydrolysate for L-lactic acid production by Bacillus coagulans JI12. BiochemicalEngineering Journal, 2014, 83:16-21.

6.        FeiGuo, Stefan Franzen, Lidan Ye, Haoming Xu, Jiali Gu, HongweiYu*. Controlling Enantioselectivity of Esterase in Asymmetric Hydrolysis ofAryl Prochiral Diesters by Introducing Aromatic Interactions, Biotechnology andBioengineering, 2014, 111(9):1729-1739.

7.        RongrongLi, Ling Jiang, Lidan Ye, Jie Lu, Hongwei Yu. OrientedCovalent Immobilization of Esterase BioH on Hydrophilic Modified Fe3O4 Nanoparticles. Biotechnologyand Applied Biochemistry,2014, 61(5):603-610.

8.        JialiGu, Min Liu, Fei Guo, Wenping Xie, Wenqiang Lu, Lidan Ye, ZhirongChen, Shenfeng Yuan, Hongwei Yu*. Virtual screening of mandelate racemasemutants with enhanced activity based on binding energy in the transition state. Enzymeand Microbial Technology, 2014, 55:121-127.

9.        WenqiangLu, Lidan Ye, Haoming Xu, Wenping Xie, Jiali Gu, Hongwei Yu*. Enhancedproduction of coenzyme Q10 by self-regulating the engineered MEP pathway in Rhodobactersphaeroides. Biotechnology and Bioengineering, 2014,111(4):761-769.

2013

1.        LidanYe#, Hongna Xu#, Hongwei Yu*. Enhanced acylationactivity of esterase BioH from Escherichia coli by directedevolution towards improved hydrolysis activity, Biochemical EngineeringJournal, 2013, 79:182-186.

2.        LidanYe, Mohammad Sufian Bin Hudari, Xingding Zhou, Dongxu Zhang, Zhi Li*, JinChuan Wu*. Conversion of acid hydrolysate of oil palm empty fruit bunch toL-lactic acid by newly isolated Bacillus coagulans JI12, AppliedMicrobiology and Biotechnology, 2013, 97(11):4831-4838.

3.        LidanYe, Xingding Zhou, Mohammad Sufian Bin Hudari, Zhi Li*, Jin Chuan Wu*.Highly efficient production of L-lactic acid from xylose by newly isolated Bacilluscoagulans C106. Bioresource Technology, 2013, 132:38-44.

4.        LidanYe, Hua Zhao, Zhi Li*, Jinchuan Wu*. Improved acid tolerance of Lactobacilluspentosus by error-prone whole genome amplification. BioresourceTechnology, 2013, 135: 459–463.

5.        XiaolianWei, Xiawei Jiang, Lidan Ye, Shenfeng Yuan, Zhirong Chen, Min Wu,Hongwei Yu*. Cloning, expression and characterization of a new enantioselectiveesterase from a marine bacterium Pelagibacterium halotolerans B2T. Journalof Molecular Catalysis B: Enzymatic, 2013, 97: 270-277.

6.        XingdingZhou, Lidan Ye, Jin Chuan Wu*. Efficient production of L-lacticacid by newly isolated thermophilic Bacillus coagulans WCP10-4with high glucose tolerance. Applied Microbiology and Biotechnology, 2013,97(10):4309-4314.

Before 2013

1.        LidanYe, Jörg Nüske, Dieter Spiteller, René Ullrich, Wilhelm Boland,Gabriele Diekert*. Fluoride- dependent conversion of organic compoundsmediated by manganese peroxidases in the absence of Mn(II). Biochemistry,2010, 49: 7264-7271.

2.        LidanYe, Anke Schilhabel, Stefan Bartram, Wilhelm Boland, Gabriele Diekert*.Reductive dehalogenation of brominated ethenes by Sulfurospirillummultivorans and Desulfitobacterium hafniense PCE-S. EnvironmentalMicrobiolgy, 2010, 12: 501-509.

3.        JiLiu, Hang Min*, Lidan Ye. The negative interaction between thedegradation of phenanthrene and tricyclazole in medium, soil and soil/compostmixture, Biodegradation, 2008, 19(5): 695-703.

4.        叶丽丹，劳佳萍，卢建平，林福呈。球毛壳菌荧光标记与重寄生现象研究。浙江大学学报（农业与生命科学版）, 2007, 33: 119-124.